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Glucomaylase

Glucoamylase is manufactured by fermentation process with selected strain belonging to Aspergillus niger. It is used to breakdown every glucosidic bond in polysaccharides like starch and glycogen thereby producing alpha-glucose.

Action
Glucoamylase hydrolyzes terminal 1,4-linked alpha-D-glucose residues from the non-reducing ends of amylose chains to release free glucose and also can hydrolyze alpha-1,6-D-glucosidic linkages in isomaltose and dextrins.

Application range
It is widely used as saccharification agent in the process of producing white oil, yellow wine, alcohol, beer, calcium lactate. It is also used in fermentation of organic acid, amino acid vitamins, and antibiotics which use glucose as fermentation culture. Other applications include decomposition of oligosaccharide, production of dextrose etc.

Activity
Solid type: 50,000 u/g,100,000u/g.
Liquid type: 100,000 u/ml,130,000 u/ml
One unit of activity is defined as the amount of glucose produced by 1g or 1ml enzyme under prescribed conditions:
Reaction time: 60 minutes
Temperature: 40℃
PH: 4.6

Characteristics
1) Yellow powder or transparent liquid
2) Temperature activity: 40-60℃. Optimum temperature: 58-60℃
3) PH activity: 3.0-5.5. Optimum pH: 4.0-4.5

Packing
Liquid: Plastic drum packing, 25 Liter net plastic drums or 1 cube meter plastic drums standard
Powder: 50Kg carton drum.

Storage
Strong sun and high temperature will cause enzyme to loose activity. Should kept in cool and dry place, avoid direct sun shining.

Enzymatic properties

Temperature activity pH activity
The standard assay of Glucoamylase was carried out at pH 4.5 with different temperatures, substrate is 4% (W/V). The optimum temperature is 58-60℃ The standard assay of Glucoamylase was carried out at 60℃with different pH. Substrate is 4% (W/V) starch. The optimum pH is 4.0-4.5
Thermostability pH stability
Real line: presence of substrate
Broken line: absence of substrate
Substrate: 30% glucose, pH: 4.5
Same enzyme quantity
Real line: presence of substrate
Broken line: absence of substrate
Substrate: 30% glucose
Same enzyme quantity

 

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